NIS-Elements AR provides a total imaging solution covering image capture, archiving, and analysis. It was developed by Nikon for the most advanced levels of research—giving the user complete control of all microscope and camera functions.
The software is geared to deliver power and maximize workflow. It handles multi-dimensional imaging flawlessly, with support for capture, display, peripheral device control, and data management and analysis of up to six dimensions (X,Y,Z,Lambda(wavelength),T, multipoint). It also offers sophisticated image processing features, such as an extremely powerful deconvolution module, exclusive one-click database capability, and Extended Depth of Focus function.
The system contributes to experiment efficiency with a database building feature developed to handle archiving and searching of large numbers of multi-dimensional image files.
Unified control of the entire imaging system offers significant benefits to users for cutting-edge research, such as live cell imaging.
NIS-Elements Ar Microscope Imaging Software
NIS-Elements performs live cell, 6D experiments quickly and flawlessly. It can organize X, Y, Z, Lambda (wavelength) and Time within one integral platform, for easier-than-ever multi-dimensional imaging. Four types of “dimensions” of acquisition – Time, Multipoint, Z-series, Wavelength (Multichannel)— can be selected.
RAM capturing enables the recording of sequences displaying very quick acquisitions lasting tens of milliseconds, by using a part of virtual memory to store temporary data of live images.
Each document (Image windows) is a three-tiered layer structure, and is therefore ideally suited for analysis. Multiple layer save is available with JPEG2000/ND2/LIM format.
NIS-Elements can work for all common file formats, such as JPEG2000, ND2, TIFF, JFF, JPG, JTF, BMP, LIM, AVI, ICS/IDS. ND2 is a special format for NIS-Elements. ND2 allows storing sequences of images acquired during ND experiments. It contains information about the hardware settings and the experiment conditions and settings.
Easy-to-use parameters for multi-dimension image operation are located on the frame of the screen.
Once a multi-dimensional image is acquired, it can be analyzed via NIS-Elements’ multiple processing functions.
A series of images from timelapse acquisition, or captured Z-stack images, can be easily converted to ND2 format. The converted images can be viewed and processed using features of NIS-Elements multidimensional document.
NIS-Elements innovative analysis capabilities include object counting, automated measurements, region of intent measurements and time measurements including image ratioing of multi-channel images.
Using the RGB or HSI color spaces, NIS-Elements can segment the image and create binary images. Using the binary image, Automatic Measurement records length, area, angle and colorimetry.
Using binary objects, NIS-Elements can automatically measure sets of length, area, density and colorimetry parameters. About 30 different object features can be measured.
The measurement is performed by, for example, directly drawing two parallel lines on the screen. Features are available from: taxonomy, counts, length, semiaxes, area, angle or profile and all output statistics and histograms can be exported to MS Excel.
There are five possible interactive profile measurements: free line, two-point line, horizontal line, vertical line, and polyline.
Classifier allows segmentation of the image pixels according to different user-defined classes, and is based on different pixel features such as intensity values, RGB values, HIS values, or RGB values ignoring intensity. The classifier enables data to be saved in separate files.
Time measurement records the average pixel intensities within defined probes during a time interval and can be performed on live camera signals.
Non-destructive measurements across time can be performed on live camera signals. Time Measurement records the average pixel intensities within defined probes during a time interval. Analysis can be performed pre- or post capture, with assurance that the specimen will live on after acquisition.
The software also handles:
NIS-Elements innovative image processing capabilities give users’ further control and functionality in their experiments. Advanced visualization methods, like capturing 3D data sets, 2D/3D data set analysis, and large image stitching can all be performed with relative ease with the software.
NIS-Elements offers a rich spectrum of mathematical morphology functions (clean, erode, dilate, open, close, smooth), morphologic separation functions, linear morphology functions, fill functions (fill holes, close holes), skeleton functions (medial axis, skeletonize, pruning) and other functions (such as: binary invert, convex hull, contour, skeletonize, homotopic marking, zones of influence, etc.).
(original, contour, threshold, zones of influence + source)
Multiple single channel images (captured with different optical filters or under different camera settings) can be merged together simply by dragging from one image to another. In addition, the combined images can be stored to a file while maintaining their original bit depths or optionally can be converted into a RGB image.
NIS-Elements’ Image Arithmetic function performs calcuations on the color channels of two images and creates a new image that is a combination of the two.
NIS-Elements includes impressive large image stitching capabilities. Here's how it works: samples are scanned automatically using a motorized XY stage with auto-focus capability and captured images are stitched into one large image. Special algorithms ensure maximum accuracy, resulting in ultra high-resolution images.
Extended Depth of Focus (EDF) is an additional software plug-in for NIS-Elements. Thanks to the EDF function, images that have been captured in a different Z-axis can be combined to create an all-in-focus image. Also, it is possible to create stereovision image & 3D surface image for a virtual 3D image.
The real time 2D deconvolution module (from AutoQuant) allows the user to observe live specimens with less out-of-focus blur. It allows faint biological processes to be observed that may otherwise be missed and increases observed signal-to-noise ratio. Available in two- and three-dimensional image stacks.
NIS-Elements makes fluorescence and brightfield photodocumentation easy and intuitive, and offers extensive data management functions. The software’s interface is designed to simplify workflow, and delivers fast image acquisition speeds via direct streaming RAM to record rapid biological events. It provides complete control over Nikon motorized microscopes and other devices with powerful functions like a zoom control linked to calibration.
NIS-Elements comes with the built-in layouts “Full Screen” and “Docked Controls.” Layouts of all windows and toolbars can be freely customized by the user and saved. Switching between layouts is achieved by a single mouse click.
Color modification can be easily set with the LUT (look-up table). Indexed-color pixels are mapped into a selected set of true color values. The histogram, threshold, gamma parameter, and brightness of RGB components are adjustable. Modifications on live image processing can be easily accomplished on the GUI.
NIS-Elements has a powerful built-in image database. The database supports image and meta-data, allowing users to create report templates or direct printed and PDF based reports. Various databases and tables can easily be created and images can be saved to the database via one simple mouse click.
In addition, filtering, sorting and multiple grouping can all be performed through the GUI's drag and drop functionality. Detail, thumbnail, icon and grid views are available on both the database image and the images on the file system.
NIS-Elements uses a sophisticated image documentation structure making it possible to achieve non-destructive archiving of image data including annotation (arrows, lines, text notes), measurement data, binary data for storing results of threshold or classification processes, and meta-data information for recording acquisition and device conditions at the time of image acquisition.
Flexibility in tailoring the software for specific applications is possible through simple built-in macro recording and the Advanced Macro Builder.
Report Generator enables the user to create customized reports containing images, database descriptions, measured data, user texts, and graphics.
PDF format files can be created directly from NIS-Elements.
All vital controls can be accessed by minimal mouse clicks.
NIS-Elements is suitable for hue adjustment, independently for each color, transforming the color image to one of the RGB or HSI components.
Color adjustments include:
All PC environments should meet the following minimum requirements:
Intel Core 2 Duo or higher
32bit Operating System: 2GB or higher
64bit Operating System: 4GB or higher
Windows Vista 32-bit Business Edition (Ar, Br and D supported)
Windows Vista 64-bit Business Edition (C, Ar, Br and D all supported)
Windows 7 32-bit Professional (Ar, Br, and D supported)
Windows 7 64-bit Professional (Ar, Br and D supported)
Note: F is ONLY supported in Vista Business 32bit (not U3 compatible) and Windows 7 Pro 32bit*Note: NIS-Elements cannot be installed on Windows XP Operating Systems.
2GB or more required for installation
1280x1024, True Color mode
For optimal volume viewing, video card must be able to support Microsoft’s Direct X (Direct Draw and Texturing) version 9.0C or higher.
Administrator Authorized Users for installing
Administrator Authorized for operating recommended but not required
Nikon Devices & CamerasMicroscopes and Accessories
Eclipse Ni-E, Ni-U
Eclipse TE2000E, TE2000E2
Eclipse TE2000-PFS (Perfect Focus System)
LiveScan SFC (Swept Field Confocal)
Eclipse LV150 Series, LV100D, LV100 POL
All Nikon motorized components (FMA, Episcopic Illuminator, Nosepiece, etc.)
Eclipse i-Series 90i
All Nikon motorized components (Focus, XY Stage, ND Filter Unit, Nosepiece, Condenser, Field Diaphragm, etc.)
Digital Imaging Heads (DDH-E (motorized) and D-DH-M)
Illumination: Intensilight E
Prairie Laser Launch with Nikon 8 Channel AOTF I/O Controller (USA only)
Photonics Instruments Mosaic and Micropoint (USA only)
LU4A Laser Unit
Agilent 4 Line Monolithic Laser Module (USA Only)
Digital Sight Cameras:
DS-Fi1, DS-5M, DS-5Mc, DS-2Mv, DS-2MBW, DS-2MBWc, DS-1QM, DS-Ri1, DS-Qi, DS-Vi1
DQC-FS EMCCD Monochrome Digital Camera
Controllers: L3, L2, U1 (32-bit only), U2, U3
Other Cameras & DevicesCameras
CoolSnap Series, Cascade Series, PVCAM compatible cameras
Only Support Non-LVSD Camera Models
Q Imaging Cameras
Retiga (models 2000R, SRV+RGB-HM-S Slider, EXi, EXi Blue, EXi-Aqua)
Rolera EM-C2, QIClick, QICAM QIC-F-CLR-12
Lumenera 1-2CB and 1-3C
Hamamatsu ORCA ER Firewire Camera, C9100-12, C9100-13, R2, ORCA-Flash2.8, Orca-Flash4.0, ORCA D2, ImagEM, ImagEM-1K
Andor Neo sCMOS, Zyla sCMOS, iXon X3, iXon+ (models 897, 888, 885), iXonEM, iKon-M, Clara, Luca S, Luca R
Prior ProScan, ProScan III, OptScan, ES10ZE
Applied Scientific Instrumentation (ASI) MS-2000
Ludl MAC 5000 / MAC 6000 Controller
Märzhäuser: Tango, LSTEP, ECO-STEP
Scientifica XY Linear Stage
Prior Filter Wheel System
Sutter Wheels, Shutters, Controllers: Lambda 10-2, 10-3, 10-B
Applied Scientific Instrumentation (ASI) FW-2000
Ludl MAC 6000 Controller
Wavelength Switching/ Excitation System:
Sutter DG 4 / DG 5 (Ultra-High Speed Wavelength Switcher)
Nikon LUSU (Laser / Shutter Unit)
Lumencor Spectra, SpectraX, Aura
Uniblitz Shutter Controller VCM-D1
Sutter: Smart Shutter Standalone Controller
Applied Scientific Instrumentation (ASI) SC-2000
Ludl MAC 6000 Controller
Nikon Motorized Z-Focus Accessory (RFA)
Prior Z-motor, ES10ZE, NIKRFK
Applied Scientific Instrumentation (ASI) MS-2000
Physik Instrumente Piezo E-665 (RS232)
All Piezo Devices Accepting Voltage
Camera Emission Splitters:
Optical Insights Dual View, Quad View
Lumen Dynamics X-Cite120, X-Cite LED1 (EXFO)
Prior Lumen Pro
Sutter Lambda XL
High Speed Analog Output Boards:
National Instruments DAQ Board (NI PCI6503, PCI6519, PCI6711, PCI6713, PCI6731, PCI6733, PCI6220, PCI6221, PCI6221(37pin), PCI6224, PCI6225, PCI6229, PCI6280, PCI6281, PCI6284, PCI6289)
Linkam TP 95, 94, 93, 92
OKOLab Microscope Incubators
Tokai Hit Microscope Incubators
NIS-Elements AR provides a total imaging solution covering image capture, archiv...